전기적 자극이 백서의 대퇴골 골형성 세포에 미치는 영향에 관한 연구
AN EXPERIMENTAL STUDY ON THE OSTEOGENESIS INDUCED BY CONSTANT DIRECT CURRENT WITH LOW AMPERAGE IN RAT
김종석, 이진용,
소속 상세정보
김종석 ( ) - 경희대학교 식품공학과
이진용 ( ) - 경희대학교 치과대학 소아치과학교실
KMID : 0358919870140010109
Abstract
결 론
저자는 생물학적 전기적 자극이 골형성세포에 미치는 영향을 관찰하기 위하여 생후 3주된
체중 200gm 내외의 백서(Sprague-Dawley strain) 28마리를 연구대상으로 하였으며, 실험군
은 21마리 대조군은 7마리로 하였다. 실험군은 대퇴골 골외막에 stainless steel로 된 음전극
을 장착시키고, 백서의 척추와 양 iliac Crest를 연결하는 선이 만나는 지점에 양전극을 피하
매식하여 전원에 연결한 후 약 7∼18μA의 직류전기를 1, 3, 6, 12, 24시간, 3일, 7일간 적응
하였다. 대조근은 실험군과 동일한 방법으로 음전극을 장착시켰으나 전원에는 연결시키지
않았다. 각 적응시간 경곽후 모든 동물을 희생시켜 광학현미경, 주사전자현미경, 투과전자현
미경으로 관찰하여 다음과 같은 결론을 얻었다.
1. 골아세포의 활성도는 12시간 및 24시간군에서 가장 높았고 그 이후 감소되었다.
2. 골양조직은 자극 개시후 6신간군에서 가장 현저하게 형성되었다.
3. 3일군의 음전극 근원심측에 신생골형성이 나타나기 시작하여, 7일군에서 가장 현저하였
다.
4. 형성된 신생골은 막내골형성에 의한 미세해면골이었다.
5. 골아세포의 배열변화는 0.937 Joule에서 나타났으며, 1.602 Joule에서 현저한 골형성이 나타났다.
#초록#
Many authors have demonstrated the ability of small amounts of electric current to
stimulate bone formation in the vicinity of a cathode. This phenomenon has been applied
to the healing of fractures in laboratory animals and humans. Surprisingly, little has
been known concerning the effects of current upon the physiologic environment of the
cell.
Several unanswered questions arose regarding for the effects induced by electric
current in light of that 'how it happened' rather than that 'what happened'. It is the
purpose of this study to determine if bone growth in rat femur could be influenced by
such a bioelectric current and also to test and evaluate the hypotheses that the author
theorized.
28 rats (Sprague-Dawley strain) weighing about 150-250gm were selected in order to
carry out this study. All surgery was done under ether anesthesia with aseptic
precautions, each animal receiving 0.15cc/100gm of soluble ganamycin sulfate
intraperitoneneally at the end of the surgical procedure.
A 0.010' stainless steel wire covered with polyethylene tube was tied at the right mid
fe-moral portion of the rat, and this wire serving as a cathode was connected to the
Constant Current Sink. Anode was implanted subcutaneously over the dorsum at the
cross section area where the vertebral column and a line connecting both iliac crests,
and then connected to the Sink. Control groups were received the same electrodes but
that those electrodes were not connected to the power supply pack.
Animals were sacrificed at 1, 3, 6, 12, 24 hours and, 3 and 7 days after the electrical
stimulation. 3 sites near the cathode were prepared for histologic findings including LM,
SEM, TEM.
Based on the present study the following conclusions can be made :
1. The increased activity of the osteoblasts peaked at 12 hours and 24 hours, and
decreased thereafter.
2. The osteoid matrix was most markedly produced in the 6 hour experimental group.
3. New bone formation was appeared in the vicinity of the cathode of all 3-day
experimental rats, and electrical stimulation results in a significant amount of new bone
formation on the femur surface of each 7-day experimental rat which did not appear in
the control groups.
4. A type of new bone formed was to be a fine cancellous bone tissue, and the newly
formed bone was produced by a process of intramembranous bone formation.
5. The total energy dissipated approximately 0.9 Joule elicited a marked arrangement
change of osteoblasts in the cellular periosteum, and a prominent bone formation was
produced at about 1.6 Joule.
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